Figure 2.

Delivery of rAAV-GFP by different routes induced diverse expression in different organs in the mice. Adult ICR mice were administered rAAV-GFP through different routes. Each ICR mouse was intravenously (i.v.) or intraperitoneally (i.p.) injected or intranasally (i.n.) or orally administered with 5 × 10¹¹ vg of recombinant AAV-GFP. After three weeks, the mice were sacrificed and the organs, including the brain, colon, heart, intestines, and liver, were collected and frozen in sections. The expression of green fluorescence protein in the different organs was detected using a fluorescence microscope (OLYMPUS IX71, DP70, TH4-100). The data presented represent one of the three mice.