Figure 4.

EV71 increased in the brain and intestines in mice transduced with rAAV-hSCARB2 and rAAV-hPSGL1. Adult ICR mice received 100 μL AAV through i.v. and oral administrations of 5 × 10¹² vg/mL of rAAV-hSCARB2 or rAAV-hPSGL1. After three weeks, the mice were or were not infected with the EV71 strain 4643 in 100 μL (1 × 10⁷ pfu/mice) via an intraperitoneal (IP) injection. (a) Seventy-two hours after infection, RNA was extracted from brain- and intestinal-tissues. The transcript of EV71 VP1 was detected by real-time RT-PCR. *P < 0.05, **P < 0.01, and ***P < 0.001 (t-test). (b) Seventy-two hours after infection, brain and intestinal tissues were homogenized in PBS. The virus titers in the supernatants of the clarified homogenates were determined by a plaque assay and expressed as plaque-forming unit per milligram (pfu/mg) tissue. *P < 0.05, ^∗∗ P < 0.01, and ^∗∗∗ P < 0.001 (t-test). (c) Frozen sections of the brain and intestines were incubated with rabbit antiserum against EV71, followed by HRP-conjugated secondary antibody. Cells that stained positive are labeled with a black arrow. The results represent one of the three mice for each group.