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. 2014 Aug 27;2014:872827. doi: 10.1155/2014/872827

Figure 2.

Figure 2

Influence of thawing on the measurement of plasma urea concentration. Blood was obtained from C57BL/6 mice for measurement of plasma urea. After centrifugation, the plasma was separated into tubes and frozen at −20°C for 4 days, followed by thawing and measurement of plasma urea by spectrophotometry. Samples were then again frozen at −20°C for 86 more days, when plasma urea was again evaluated. Values are expressed in mg/dL; (∗) statistically significant differences at P < 0.05.