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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Cancer Res. 2014 May 16;12(9):1267–1282. doi: 10.1158/1541-7786.MCR-13-0652-T

Figure 2.

Figure 2

Detection of GM-CSFRα in the cytoplasm and nucleus of chronic lymphocytic leukemia (CLL) cells. (A, B) Confocal microscopic images of freshly isolated CLL cells that were cytospun and fixed on glass slides; the slides were stained with PE-conjugated anti-GM-CSFRα antibodies, as described in the Methods section. As shown (×400), GM-CSFRα is present in the cytosol and to a lesser extent in the nucleus of CLL cells. (C) Cytoplasmic and nuclear fractions were extracted from CLL cells of 2 patients and analyzed by Western immunoblotting using anti-GM-CSFRα antibodies. Adequate fractionation of cytoplasmic and nuclear extracts was confirmed using anti-S6 ribosomal protein and anti-lamin B1 antibodies. As shown, GM-CSFRα was detected in the nucleus of CLL cells.