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. Author manuscript; available in PMC: 2015 Aug 25.
Published in final edited form as: Mol Cell Endocrinol. 2014 Jul 6;394(0):47–58. doi: 10.1016/j.mce.2014.06.021

Figure 3.

Figure 3

The mutations in the basic regions primarily affect the DNA-binding properties of Met and FISC. (A) Transfection assay with a JHRE-driven luciferase reporter. Aag2 cells were transfected by the 4×JHRE1-luc reporter construct and expression vectors for the derivatives of Met and FISC. Transfected cells were treated with 10−6 M JH-III or ethanol. (B) Transfection assay with a UAS-driven luciferase reporter. Aag2 cells were transfected by the UAS×4-188-cc-Luc reporter construct and expression vectors for the derivatives of Met and FISC. All the Met derivatives were expressed as fusions to the GAL4 DNA-binding domain.