Figure 3.
The mutations in the basic regions primarily affect the DNA-binding properties of Met and FISC. (A) Transfection assay with a JHRE-driven luciferase reporter. Aag2 cells were transfected by the 4×JHRE1-luc reporter construct and expression vectors for the derivatives of Met and FISC. Transfected cells were treated with 10−6 M JH-III or ethanol. (B) Transfection assay with a UAS-driven luciferase reporter. Aag2 cells were transfected by the UAS×4-188-cc-Luc reporter construct and expression vectors for the derivatives of Met and FISC. All the Met derivatives were expressed as fusions to the GAL4 DNA-binding domain.