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. Author manuscript; available in PMC: 2015 Aug 25.
Published in final edited form as: Mol Cell Endocrinol. 2014 Jul 6;394(0):47–58. doi: 10.1016/j.mce.2014.06.021

Figure 5.

Figure 5

The binding affinities of the AaMet-AaFISC complex to various JHREs. (A) Dissociation constant (Kd) of the binding of Met-FISC to MFBS1 was measured by gel-shift assays. Details were described in the Experimental Procedures. (B) Scatchard analysis of the binding of AaMet-AaFISC to MFBS1. The Kd value was calculated from the slope of the regression line. (C) Comparison of the binding affinities of AaMet-AaFISC to various JHREs. Kd was shown as mean±SD, n=3. ND, Kd was not determined because the DNA binding could not be detected.