Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Dev Biol. 2014 Jul 27;394(1):156–169. doi: 10.1016/j.ydbio.2014.07.013

Figure 4.

Figure 4

The over expression of PPE genes can alter the accumulation of PCP proteins. A and A’ are from the same ptc-Gal4/+; UAS-frtz/act-Vang-YFP wing. A is from outside the ptc domain and A’ from inside the domain. Vang-YFP immunostaining is shown. Outside the ptc domain Vang accumulates in the typical proximal/distal zig-zag pattern. Inside the ptc domain Vang accumulation is more punctate and is preferentially found on the anterior/posterior sides of cells. B and B’ are from the same ptc-Gal4/+; UAS-frtz wing. B is from outside the ptc domain and B’ from inside the domain. Endogenous Pk accumulation is shown by immunostaining. Outside the ptc domain Pk accumulates in the typical proximal/distal zig-zag pattern. Inside the ptc domain Pk accumulation is more punctate and is preferentially found on the anterior/posterior sides of cells. C shows a ptc-Gal4/dsh-myc; UAS-frtz/+ wing that was immunostained with anti-Myc antibody to show the accumulation of Dsh-myc. E shows a ptc-Gal4 UAS-HA-in UAS-fy-GFP/+ wing immunostained to show the accumulation of the endogenous Pk protein. Note the increased Pk accumulation and altered zigzag accumulation. E shows a ptc-Gal4 UAS-HA-in UAS-fy-GFP/+ wing immunostained to show the accumulation of the endogenous Stan protein. Note the loss of the distinct proximal/distal zigzag in the ptc domain. F shows a ptc-Gal4/+; UAS-frtz-GFP/+ wing immunostained to show the accumulation of the endogenous Pk protein. Note the slightly increased accumulation of Pk and the loss of the proximal/distal zigzag in the ptc domain.