The over expression of one PPE protein affects the accumulation of the others. A, B and C
show a ptc-Gal4/UAS-HA-in; ubi-myc-frtz-GFP/+ wing immunostained for both
In (red) and GFP (green-Frtz). This ubi-myc-frtz-GFP transgene gives
variegated expression. D shows the quantitation of GFP (Frtz) staining inside (2) and
outside (1) of the ptc domain. (Note the quantitation was done on the
peripheral accumulation in cells that expressed high levels of Frtz). The increase in Frtz
accumulation was significant (p<0.05). E, F and G shows a
ptc-Gal4/UAS-fy-Flag-ollas; ubi-myc-frtz-GFP/+ wing immunostained for
both Ollas (red-Fy) and GFP (green-Frtz). H shows the quantitation of GFP (Frtz) staining
inside (2) and outside (1) of the ptc domain. The decrease in Frtz
accumulation was significant (p<0.05). I, J and K show the results of Western blots
of wing disc samples where the expression of UAS-HA-in and
UAS-myc-frtz were driven either singly or together using
ptc-Gal4. I and J show the quantitation and K the blot. M, N and L show
the results of Western blots of wing disc samples where the expression of
UAS-fy-Flag-Ollas and UAS-myc-frtz were driven either
singly or together using ptc-Gal4. L and M show the quantitation and N
the blot. O, P and Q show the results of Western blots of wing disc samples where the
expression of UAS-HA-in and UAS-fy-GFP were driven
either singly or together in varying doses using ptc-Gal4. O and P show
the quantitation and Q the blot.