Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: Mol Cancer Res. 2014 Jun 19;12(9):1334–1343. doi: 10.1158/1541-7786.MCR-14-0049

S100A9 suppresses RhoC expression. RhoC expression in MCF7 cells stably expressing a vector control, or Id1, or Id1 and S100A9 was determined by quantitative RT-PCR (A) and immunoblot (B). Id1 expression in MCF7 cells increased RhoC expression (A and B). Expression of S100A9 cDNA reversed the increase of RhoC expression induced by Id1 expression (A and B). (C) Immunoblots of Id1, S100A9 and RhoC in MDA-MB-436 cells expressing a control shRNA or Id1 shRNA. Knockdown of Id1 expression in MDA-MB-436 cells increased S100A9 expression and decreased RhoC expression. (D) RhoC expression in MCF7 cells expressing a vector control, or Id1, or Id1 and RhoC shRNA 1, or Id1 and RhoC shRNA 2. (E–H) RhoC mediated the Id1 functions in migration and invasion. MCF7 cells stably expressing a vector control, or Id1, or Id1 and RhoC shRNA 1, or Id1 and RhoC shRNA 2 were subjected to migration (E) and invasion (F) assays. Id1 expression in MCF7 cells increased cell migration and invasion (E and F). Knockdown of RhoC expression reversed the migratory and invasive phenotypes induced by Id1 (E and F). MCF7 cells stably expressing a vector control, or Id1, or Id1 and S100A9, or Id1 plus S100A9 and RhoC were subjected to migration (G) and invasion (H) assays. S100A9 expression decreased the cell migratory and invasive phenotypes induced by Id1 (G and H). Expression of RhoC rescued the suppressive phenotype of S100A9 in migration and invasion (G and H).

S100A9 suppresses RhoC expression. RhoC expression in MCF7 cells stably expressing a vector control, or Id1, or Id1 and S100A9 was determined by quantitative RT-PCR (A) and immunoblot (B). Id1 expression in MCF7 cells increased RhoC expression (A and B). Expression of S100A9 cDNA reversed the increase of RhoC expression induced by Id1 expression (A and B). (C) Immunoblots of Id1, S100A9 and RhoC in MDA-MB-436 cells expressing a control shRNA or Id1 shRNA. Knockdown of Id1 expression in MDA-MB-436 cells increased S100A9 expression and decreased RhoC expression. (D) RhoC expression in MCF7 cells expressing a vector control, or Id1, or Id1 and RhoC shRNA 1, or Id1 and RhoC shRNA 2. (E–H) RhoC mediated the Id1 functions in migration and invasion. MCF7 cells stably expressing a vector control, or Id1, or Id1 and RhoC shRNA 1, or Id1 and RhoC shRNA 2 were subjected to migration (E) and invasion (F) assays. Id1 expression in MCF7 cells increased cell migration and invasion (E and F). Knockdown of RhoC expression reversed the migratory and invasive phenotypes induced by Id1 (E and F). MCF7 cells stably expressing a vector control, or Id1, or Id1 and S100A9, or Id1 plus S100A9 and RhoC were subjected to migration (G) and invasion (H) assays. S100A9 expression decreased the cell migratory and invasive phenotypes induced by Id1 (G and H). Expression of RhoC rescued the suppressive phenotype of S100A9 in migration and invasion (G and H).