Table 3. Unique Sphingolipid and Ceramide Species in Normotensive and Hypertensive Male DBA/2J Aqueous Humor.
| Lipid Speciesa | Theoretical m/zb |
m/z | Error (Da) |
Average lipid amount (pmol per species/μg protein)c |
LIPIDMAPS ID | PUBCHEM ID | HMDB Accessiond |
|---|---|---|---|---|---|---|---|
| Sphingomyelin | |||||||
|
| |||||||
| Normotensive Aqueous Humor | |||||||
|
| |||||||
| SM(d18:0/15:0)e | 691.6 | 691.9 | 0.3 | 6.9 | LMSP03010091 | 123068802 | |
| SM(d20:0/24:1) | 843.7 | 843.2 | −0.5 | 1.7 | LMSP03010088 | 123068799 | |
|
| |||||||
| Hypertensive Aqueous Humor | |||||||
|
| |||||||
| SM(d16:1/16:0)e | 675.5 | 675.5 | −0.1 | 12.2 | LMSP03010035 | 123068746 | |
| SM(d18:1/22:0) | 787.7 | 787.2 | −0.5 | 239.8 | LMSP03010006 | 7850649 | |
| SM(d16:1/17:0) | 689.6 | 689.9 | 0.3 | 1.5 | LMSP03010037 | 123068748 | |
| SM(d16:1/20:1)e | 729.6 | 729.3 | −0.3 | 0.7 | LMSP03010048 | 123068759 | |
| SM(d17:1/22:0) | 773.7 | 773.8 | 0.1 | 23.0 | LMSP03010066 | 123068777 | |
| SM(d18:0/14:0) | 677.6 | 676.6 | −1.0 | 6.2 | LMSP03010030 | 49703632 | |
| SM(d18:0/24:1(15Z)) | 815.7 | 815.7 | 0.0 | 18.9 | LMSP03010023 | 7850657 | |
| SM(d18:1/20:1)e | 757.6 | 756.6 | −1.0 | 19.8 | LMSP03010059 | 123068770 | |
|
| |||||||
| Sphingoid base | |||||||
|
| |||||||
| Hypertensive Aqueous Humor | |||||||
|
| |||||||
| Penaresidin Ae | 330.3 | 330.3 | 0.0 | 1.2 | LMSP01080045 | 74382694 | |
|
| |||||||
| Ceramides | |||||||
|
| |||||||
| Normotensive Aqueous Humor | |||||||
|
| |||||||
| Cer(t18:0/26:0) | 694.7 | 694.8 | 0.1 | 7.4 | LMSP02030005 | 85297410 | |
| CerP(d18:1/24:0) | 728.6 | 727.7 | −0.9 | 1.4 | LMSP02050008 | 7850645 | HMDB10704 |
| SM(d16:1/18:0)e | 701.6 | 702.3 | 0.8 | 2.7 | LMSP03010042 | 123068753 | |
| SM(d18:1/15:0)e | 687.5 | 687.9 | 0.4 | 5.1 | LMSP03010038 | 123068749 | |
| SM(d18:1/16:1) | 699.5 | 700.0 | 0.5 | 7.2 | LMSP03010041 | 123068752 | |
| SM(d18:2/15:0) | 685.5 | 686.2 | 0.7 | 1.9 | LMSP03010036 | 123068747 | |
|
| |||||||
| Hypertensive Aqueous Humor | |||||||
|
| |||||||
| Cer(d18:1/22:0) | 620.6 | 621.2 | 0.6 | 0.5 | LMSP02010008 | 7850626 | HMDB04952 |
| SM(d16:1/20:1)e | 727.6 | 728.1 | 0.5 | 7.8 | LMSP03010048 | 123068759 | |
| SM(d16:1/24:1) | 783.6 | 784.3 | 0.6 | 0.2 | LMSP03010071 | 123068782 | |
| SM(d18:0/12:0)e | 647.5 | 648.1 | 0.6 | 6.0 | LMSP03010079 | 123068790 | |
| SM(d18:0/18:1(9Z)) | 729.6 | 730.1 | 0.5 | 0.1 | LMSP03010031 | 49703633 | |
| SM(d18:0/26:1(17Z)) | 841.7 | 842.3 | 0.6 | 0.1 | LMSP03010025 | 7850659 | |
| Cer(d16:1/22:0) | 592.6 | 593.2 | 0.7 | 13.0 | LMSP02010016 | 123068725 | |
| Cer(d18:0/24:1(15Z)) | 648.6 | 649.3 | 0.7 | 0.1 | LMSP02020011 | 7850635 | HMDB11769 |
| Cer(d18:1/18:1(9Z)) | 562.5 | 563.3 | 0.8 | 47.0 | LMSP02010003 | 4266351 | HMDB04948 |
| Cer(d18:1/26:1(17Z)) | 674.6 | 675.6 | 1.0 | 2.0 | LMSP02010010 | 7850628 | HMDB04954 |
| SM(d18:0/17:0)e | 717.6 | 718.1 | 0.5 | 2.9 | LMSP03010046 | 123068757 | |
| SM(d18:1/25:0) | 827.7 | 828.1 | 0.4 | 2.7 | LMSP03010027 | 49703629 | |
The lipid species identification is based on Lipidmaps database, customized and used as a .csv file for bioinformatic analyses with the MZmine 2.9 program.
The theoretical mass by charge ratio (m/z) presented is in positive ion mode [M+H]+ for sphingomyelin and sphingoid base. Whereas negative ion mode [M-H]− has been presented for sphingoid base-1-phosphate and ceramide. Error in Da has been provided from theoretical data.
Average standard normalized dataset is presented here. Species that are known bonafide lipids of non-mammalian origin were excluded. However, where the origin in mammalian or human is unclear those entities were retained.
The only lipids species in this table that was found to have an HMDB identifier (HMDB10704, HMDB04952, HMDB11769, HMDB04948, HMDB04954), following details have been reported: Localization: extracellular membrane and membrane component, functions: membrane integrity, cell signaling, energy source and energy storage.
Lipids common to females and males.