Figure 5.

mTOR inhibitors sensitize ovarian cells to MPL-induced cell death. A. Cells pre-treated with 50 nM rapamycin (Rap. mTOR inhibitor, autophagy inducer) and 1 nM everolimus (Eve., ROD001, mTOR inhibitor, autophagy inducer), 30 min. prior to treatment with MPL (10 μM) under the cell culture conditions. Each concentration was tested in replications of 8 and each experiment was repeated 3 times. Data represent mean ± SEM from three independent experiments combined. B. Cells were grown in six-well tissue culture plates under standard cell culture conditions, pre-treated (30 min) with rapamycin and everolimus followed by MPL (0 and 10 μM) for 72 h. Cells were then stained with giemsa (10% in PBS), washed and photographed under Leica DM IRB light microscope (magnification 40x). Black arrows show vacuoles. A representative from three independent experiments has been shown.