Table 5.
Changes in the expression levels of miRNA target genes in GSCs treated with 450 μM TMZ in the presence or absence of 1 mg/ml and 2 mg/ml OLE
| TP53 | OCT-4 | SOX2 | BCL2 | c-myc | c-met | |
|---|---|---|---|---|---|---|
| Untreated | ||||||
| 2^(-Avg.(Delta(Ct)) | 0.014418 | 0.02039 | 0.184795 | 0.042926 | 0.014458 | 0.011454 |
| 2 mg/ml OLE | ||||||
| 2^(-Avg.(Delta(Ct)) | 0.002418 | 0.002828 | 0.013831 | 0.010352 | 0.002418 | 0.002418 |
| Fold Change | 0.1677 | 0.1387 | 0.0748 | 0.2411 | 0.1672 | 0.2111 |
| 95% CI | (0.00001, 0.58) | (0.00001, 0.46) | (0.00001, 0.29) | (0.00001, 0.98) | (0.00001, 1.01) | (0.00001, 1.09) |
| * P value | 0.314169 | 0.227929 | 0.175984 | 0.351612 | 0.341963 | 0.302438 |
| 2 mg/ml OLE + 450 µM TMZ | ||||||
| 2^(-Avg.(Delta(Ct)) | 0.004743 | 0.002418 | 0.011502 | 0.007557 | 0.000975 | 0.000975 |
| Fold Change | 0.329 | 0.1186 | 0.0622 | 0.176 | 0.0675 | 0.0851 |
| 95% CI | (0.00001, 1.10) | (0.00001, 0.42) | (0.00001, 0.26) | (0.00001, 0.70) | (0.00001, 0.39) | (0.00001, 0.41) |
| * P value | 0.329112 | 0.220026 | 0.187845 | 0.353684 | 0.341256 | 0.29579 |
| 450 µM TMZ | ||||||
| 2^(-Avg.(Delta(Ct)) | 0.121751 | 0.121751 | 0.133046 | 0.155609 | 0.121751 | 0.121751 |
| Fold Change | 8.4444 | 5.9711 | 0.72 | 3.625 | 8.421 | 10.6295 |
| 95% CI | (0.00001, 28.07) | (0.00001, 20.13) | (0.00001, 2.91) | (0.00001, 14.44) | (0.00001, 50.50) | (0.00001, 53.82) |
| *P value | 0.209763 | 0.224161 | 0.23702 | 0.484775 | 0.371175 | 0.681602 |
*
Evaluated with an independent sample T-test using RT2 Profiler PCR Array Data Analysis.