Figure 2. FR-β is expressed primarily on the CD14^highCD16⁻ subset of human peripheral blood monocytes.

Normal human PBMCs were stained simultaneously with a mAb to FR-β (solid lines) or human IgG1 isotype control (dashed lines) and both anti-CD14 (PE-labeled) and anti-CD16 (TriColor-labeled) antibodies. Expression of FR-β on the different monocyte subsets was determined by three-color flow cytometry. Data shown are representative of three independent studies.