Skip to main content
. 2014 Oct;96(4):601–609. doi: 10.1189/JLB.2A0414-192R

Figure 4. Role of renin and Hpr in enhancing HIV replication through their proteolytic activities.

Figure 4.

(A) Control TCs and TCs overexpressing Hpr were either pulsed with HIV (H) or treated with buffer and then incubated in media containing either buffer or renin (R, 1 nM), aliskiren (A, 1 μM), or renin+aliskiren for 24 h (n=3). Protein blots were probed for Gag protein; the same blots were reprobed for actin. Representative gels are displayed. Cumulative densitometric data of Gag protein expression under control and experimental conditions are shown in the bar graph (n=3). *P < 0.05, compared to C and H/A; **P < 0.01, compared to H/Hpr; ***P < 0.01 compared H alone; and ****P < 0.01, compared to H alone. (B) Lysates of pr55 gag polyprotein-expressing TCs (100 μL) were incubated in medium containing buffer (C) or lysates of TCs overexpressing Hpr (600 μL), renin (1 nM), renin+aliskiren (Alis, 1 μM), or Hpr+Alis for 60 min (n=3). Aliquots of media were collected for p24 ELISA. Results (mean ± sd) are from 3 sets of experiments. *P < 0.001, compared to C; **P < 0.01, compared to C; ***P < 0.01, compared to Ren; and ****P < 0.01, compared to Hpr. (C) Aliquots of Agt were incubated in medium containing lysates of Hpr-expressing TCs (600 μL) and renin (1 nM) for 30 min (n=3). Subsequently, aliquots of media were collected for Ang I ELISA. Cumulative data are shown in the bar graph. *P < 0.001, compared to C.