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. 2014 Sep 15;127(18):4078–4088. doi: 10.1242/jcs.154716

Fig. 5.

Fig. 5.

ER stress-induced autophagy selectively targets the ER and does not require the core autophagy machinery. (A) Schematics of the ER-localized reporters, all of which expose Pho8Δ60 to the cytosol. (B) Fold change of Pho8 activity of reporters for autophagy of cytosol (cyto-Pho8Δ60), mitochondria (mito-Pho8Δ60) and ER (Sec63–Pho8Δ60, Sec66–Pho8Δ60, Rtn1–Pho8Δ60 and Yop1–Pho8Δ60) upon nitrogen starvation in wild-type (WT) cells (blue bars) and Δatg7 cells (dark red bars). Data are mean±s.e.m., n = 3. (C) Fold change of Pho8 activity of reporters for autophagy of cytosol (cyto-Pho8Δ60) and ER (Sec66–Pho8Δ60, Rtn1–Pho8Δ60 and Yop1–Pho8Δ60) upon nitrogen starvation in WT (blue bars) and PMSF-treated Δpep4 cells (orange bars). (D) Fold change of Pho8 activity of the same reporters as in B upon tunicamycin treatment. Data are mean±s.e.m., n = 5. (E) Fold change of Pho8 activity of the same reporters as in C upon tunicamycin treatment.