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. 2014 Aug 1;3(9):803–814. doi: 10.1242/bio.20148771

Fig. 6. Rab8, Rab13, and Rab36 function as essential mediators for neurite outgrowth downstream of Rab35.

Fig. 6.

(A) Inhibition of active Rab35-enhanced neurite outgrowth by depletion of Rab8, Rab13, or Rab36. PC12 cells expressing EGFP together with mStr + siControl, mStr–Rab35-Q67L + siControl, mStr–Rab35-Q67L + siRab8, mStr–Rab35-Q67L + siRab13, or mStr–Rab35-Q67L + siRab36 were fixed after NGF stimulation for 36 hr. (B) Inhibition of active Rab35-enhanced neurite outgrowth by expression of a dominant negative mutant of Rab8, Rab13, or Rab36. PC12 cells expressing mStr + EGFP, mStr–Rab35-Q67L + EGFP, mStr–Rab35-Q67L + EGFP–Rab8-T22N, mStr–Rab35-Q67L + EGFP–Rab13-T22N, or mStr–Rab35-Q67L + EGFP–Rab36-T71N were fixed after NGF stimulation for 36 hr. Scale bars: 30 µm. (C) Total neurite length (mean and SE) of mStr + siControl-expressing, mStr–Rab35-Q67L + siControl-expressing, mStr–Rab35-Q67L + siRab8-expressing, mStr–Rab35-Q67L + siRab13-expressing, and mStr–Rab35-Q67L + siRab36-expressing PC12 cells after NGF stimulation for 36 hr (n>100). (D) Total neurite length (mean and SE) of mStr + EGFP-expressing, mStr–Rab35-Q67L + EGFP-expressing, mStr–Rab35-Q67L + EGFP–Rab8-T22N-expressing, mStr–Rab35-Q67L + EGFP–Rab13-T22N-expressing, and mStr–Rab35-Q67L + EGFP–Rab36-T71N-expressing PC12 cells after NGF stimulation for 36 hr (n>100).