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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Biochem J. 2014 Aug 15;462(1):163–171. doi: 10.1042/BJ20140220

Table 1.

Rate constants determined from transient multiple-turnover experiments with AKR1D1

Parameters Testosterone Aldosterone Cortisone Cholestenone 7α-Hydroxycholestenone
Aburst (%) 45 ± 1 89 ± 1 53 ± 1 No burst No burst
kburst (s−1) 0.71 ± 0.03 1.98 ± 0.05 1.39 ± 0.04
kss (s−1) 0.050 ± 0.002 0.094 ± 0.002 0.104 ± 0.002 0.0060 ± 0.0002 0.0160 ± 0.0002
kcat (s−1) 0.14 ± 0.03 0.15 ± 0.01 0.195 ± 0.002 0.0100 ± 0.0007 0.033 ± 0.002

kcat was determined by steady-state analysis [19]. Values are means± S.E.M.