Fig. 1. Berberine inhibited FBS-induced migration of HASMCs. (A) HASMCs were pretreated with or without berberine (25, 50, 100 μM) for 24 h, then cell migration of HASMCs through matrigel basement membrane toward 10% FBS DMEM was analyzed using a modified Boyden chamber method. Migrated cells on the lower membrane surface were stained with crystal violet, and then eluted in 10% acetic acid. Migratory capacity was shown as the relative optical density in comparison to untreated cells. (B) Protein expression of MMP-2, MMP-9, u-PA in HASMCs treated with 10% FBS or not was assessed by Western blotting. Densitometry of different groups was normalized to β-actin.*P ＜ 0.05 compared with the serum-free group, ＃P ＜ 0.05 compared with the serum treated group.
