Fig. 3. Oxidative stress induced by ZBTB3 knockdown causes apoptosis. (A) The effects of ZBTB3 knockdown on accumulation of intracellular H₂O₂ levels in several cancer cells measured using an oxidation-sensitive dye DCFH-DA. Representative images are shown (left) and values represent mean ± SD of independent experiments performed three times; *P ＜ 0.05 and ^#P ＜ 0.05. (B) The effects of ZBTB3 knockdown on cellular GSH levels in several cancer cell lines. Values represent mean ± SD of independent experiments performed three times; *P ＜ 0.05. (C-E) The effects of ZBTB3 knockdown on antioxidant activities such as GPx, SOD, and CAT in cancer cells. Values represent mean ± SD of independent experiments performed three times; *P ＜ 0.05. (F) The effects of antioxidants on ZBTB3 knockdown-induced apoptosis. (G) The effects of the antioxidant NAC on viability in cells with ZBTB3 knocked down. Values represent mean ± SD of independent experiments performed three times; *P ＜ 0.05 and ^#P ＜ 0.05.