Fig. 1. hSP56 expression exhibited profound effects on prostate cancer cell growth. (A) Establishment of stable cell lines, PC-3/hSP56 and LNCaP/hSP56^KD cells. (B, C) Cell growth curves of PC-3 cells and derivatives (B), or LNCaP cells and derivatives (C) in anchorage-dependent liquid cultures. (D) Soft agar colony-forming assay. Number of colonies and their size were analyzed using the ImageJ software (NIH). Similar results were obtained in repeated experiments. Scale bar, 500 μm. (E) In vivo tumorigenicity experiment. (F) Pictures of representative mice taken at week 9. The site of injection is marked with dotted circle in one of the pictures.