Fig. 1. Effect of OroA on PMA, TNF-α and IL-1β-induced EPCR shedding. The effects of various concentrations of OroA on PMA (1 μM, 1 h)-induced EPCR shedding were monitored by measurement of sEPCR (A) or cellular EPCR on HUVECs (B). (C and D) The same as A and B, except that HUVECs were incubated with TNF-α (25 ng/ml for 1 h, white bar) or IL-1β (25 ng/ml for 1 h, black bar). Results indicate the mean ± SEM of three separate experiments. **P ＜ 0.01 vs. PMA alone (A, B) or TNF-α/IL-1β alone (C, D).
