Fig. 2. HDAC3 activity is necessary for the binding of modified histones to the MDR1 promoter sequences. (A) 48 h after transfection with the indicated construct to SNU387R or Mlame3MR cells, Western blot was performed. Cell lysates isolated from Malme3M or SNU387 cells were also subjected to Western blot analysis. (B) 48 h after transfection, cell lysates were immunoprecipitated with the indicated antibody (2 μg/ml), followed by ChIP assays.
