Fig. 4. The localization of HDAC3 deletion constructs. (A) The indicated cell line was transiently transfected with the indicated HDAC3-Myc/His construct. 48 h after transfection, immunofluorescence staining was performed, as described. SNU387R and Malme3MR cell lines transfected with full-length HDAC3 were also subjected to immunofluorescence staining employing only isotype-matched secondary antibody. (B) Cell lysates from each fraction were subjected to Western blot analysis.
