Fig. 2. Profiles on the binding of serum proteins to collagen and fibrin. (A) The amount of total serum protein binding to collagen and fibrin matrices was determined by MicroBCA assay. (B) Western blots for fibronectin and vitronectin adsorbed to collagen and fibrin matrices from serum. Recovered serum proteins adsorbed to the matrices were separated via SDS–PAGE. (C) Serum fibronectin and vitronectin bound to fibrin was measured using the band intensities in Western blot analyses. *Serum fibronectin adsorbed to fibrin was significantly different from that of collagen (ANOVA, P ＜ 0.05).
