Fig. 3. MAGEB16 and Mageb16 mRNA expression in HeLa and B16 cells after treatment with 5'-aza-CdR. (A) HeLa cells were treated with DMSO (0) or 5'-aza-CdR at the designated concentrations (0.1, 0.5, 1, 5 or 10 μmol/L) and were evaluated for MAGEB16 mRNA expression. (B) B16 cells were treated with DMSO (0) or 5'-aza-CdR at the designated concentrations (0.1, 0.5, 1, 5 or 10 μmol/L) and were evaluated for Mageb16 mRNA expression. The levels of mRNA were determined by QRT-PCR on isolated RNA. The testis values were used as 1 arbitrary unit=100%. (C) The methylation status of the MAGEB16 CGIs in HeLa cells treated by 10 μmol/L 5'-aza-CdR. (D) The methylation status of the Mageb16 CGI in B16 cells treated by 10 μmol/L 5'-aza-CdR. Bisulfite genomic sequencing displayed extensive demethylation in treated cells.