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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Nature. 2012 Aug 30;488(7413):670–674. doi: 10.1038/nature11290

Fig. 2. PKR is important for inflammasome activation.

Fig. 2

a-b. LPS-primed PKR+/+ or PKR-/- macrophages were stimulated as indicated. c. PKR+/+ macrophages were stimulated or treated with 2-AP or C13H8N4OS (CNS) as indicated. d-e. PKR+/+ or PKR-/- mice (n=5) were injected with live E. coli. f-g. HEK293A cells were transfected as indicated. Caspase-1 activation and IL-1β cleavage were assessed by Western-blot. Data are representative of at least three independent experiments. Levels of IL-1β, IL-18, HMGB1, and IL-6, in the supernatant (b) or serum (d) were determined by ELISA. Peritoneal lavage fluid was collected and neutrophil content measured by flow cytometry (e). Data shown are means ± SD. #, p<0.05 vs. wild-type infected groups.