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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Nature. 2012 Aug 30;488(7413):670–674. doi: 10.1038/nature11290

Fig. 3. PKR physically interacts with NLRP3 and facilitates inflammasome activation.

Fig. 3

a-b. Immunoprecipitation (IP) and Western-blot (WB) analysis of the physical interaction of PKR and NLRP3 in cell-free system using recombinant proteins (a) or LPS-primed macrophages stimulated with ATP or treated with of 2-AP or C13H8N4OS (CNS) as indicated (b). c. The NLRP3 inflammasome was reconstituted using recombinant proteins and ATP/Poly I:C as indicated. Caspase-1 activity was measured by hydrolysis of WEHD-pNA. d. PKR+/+ macrophages were stimulated or treated with 2-AP as indicated. Cell lysates were subjected to gel-filtration chromatograph and western-blot. Results are representative of three independent experiments.