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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Nature. 2012 Aug 30;488(7413):670–674. doi: 10.1038/nature11290

Fig. 4. PKR regulates NLRP1, AIM2, NLRC4 inflammasome activation.

Fig. 4

a. HEK293A cells were transfected as indicated. The physical interaction of PKR and myc-tagged proteins was analyzed by Immunoprecipitation (IP) and Western-blot (WB) analysis. b-d. LPS-primed (b) or unprimed (c, d) PKR+/+ or PKR-/- macrophages were stimulated with Anthrax lethal toxin (LT), or transfected with Poly (dA:dT), or infected with S. Typhimurium (S.T). MOI, multiplicity of infection. e. PKR+/+ macrophages were stimulated or treated with 2-AP as indicated. f. HEK293A cells were transfected as indicated. Caspase-1 activation, IL-1β cleavage, and HMGB1 secretion were assessed by Western-blot. Results are representative of at least three independent experiments.