Table 1.

A summary of information for each peptide analyzed by nLC-PC-IDMS-SRM. The corresponding International Protein Index (IPI) database identifier and mass is listed beneath the protein name. The heavy labeled amino acid in the peptides monitored for absolute quantification are in bold and underlined. The number in the peptide label corresponds to the starting amino acid location of the species in the protein. The precursor mass for the natural or endogenous species (NAT) and the SIL internal standard are all 2+ charge state parent ions. The transitions included were used to monitor protein abundance in the plasma and tissue samples with an exception of the y³ transition for OVOS2₇₇₉ and OVOS2₇₉₂ in which these transitions are marked with an asterisk.

Protein Mass (Da)	Peptide	Label	NAT m/z	SIL m/z	Transition Ions	Amount SIL on column	Collision Energy (V)
OVOS2 (IPI00587419.3) 184950.5	AVDQSVFLLQPER	OVOS2779	751.4041	754.9127	y4, y3, b9	2.5 fmol	28.0
	ELSAESVYYR	OVOS2792	608.7959	611.8028	y8, y6, y3	2.5fmol	22.9
	AEVLSPEGNTAR	OVOS21017	622.3175	625.8261	y8, b3, b5	2.5 fmol	23.4
Vitellogenin-1 (IPI00591843) 210630.1	NIEDLAASK	Vit918	480.7535	484.2620	y7, b2, a2	50 fmol	18.3
Transthyretin (IPI00591282.3) 16309.3	AADGTWQDFATGK	TTR58	684.3149	689.3286	y8, y7, y3, y2	25 fmol	25.6
	VEFDTSSYWK	TTR93	631.2904	636.3040	y7, y5, b2, a2	25 fmol	23.7