FIG. 7.

Oxidative stress induced by either RNS or ROS perturbs chromatin trafficking of c-Fos and Fra-1. (A) Chromatin and free fractions were prepared from quiescent C10 cells that had been stimulated for 6 h with medium containing 10% FBS alone or containing SIN-1 (1 mM) or GO (15 U/ml) without (−) or with (+) the addition of catalase (2,000 mU/ml) and analyzed by immunoblotting for c-Fos or Fra-1. (B) Serum-stimulated C10 cells were treated with GO as described above for 4 h, and chromatin lysates were analyzed by immunoblotting for total c-Fos as before or for c-Fos specifically phosphorylated on Thr-325 with a phosphospecific antibody. (C) Serum-starved C10 cells were treated with medium containing 10% FBS with or without GO (15 mU/ml) for 6 h prior to fixation and immunostaining for either c-Fos or Fra-1 (green). Nuclei were stained with propidium iodide (red).