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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Immunother. 2014 Oct;37(8):407–415. doi: 10.1097/CJI.0000000000000052

Figure 4. Canine HER2-CAR T cells recognize human and canine HER2.

Figure 4

(A) FACS analysis for HER2 expression of A549, MDA, MDA-hHER2, and MDA-cHER2. (B) Detection of HER2 expression in MDA, MDA-hHER2, and MDA-cHER2 by qRT-PCR using species-specific HER2 primers (mean of triplicates ± SEM) (C). Cytotoxicity assays using NT (white) and HER2-CAR T cells (solid) as effectors, and A549, MDA, MDA-hHER2, MDA-cHER2, and canine T cells as targets. Only HER2-positive targets (A549, MDA-hHER2, and MDA-cHER were killed by HER2-CAR T cells (n=3, mean ± SEM, *p<0.001).