FIG. 4.

BLM-hp150 colocalization is cell cycle dependent. (A) Fluorescence-activated cell sorter analysis of HeLa cells, which were either unsynchronized (control) or synchronized at different cell cycle stages: G₁/S, early S, and G₂/M (see Materials and Methods). (B) HeLa cells blocked at the G₁/S phase boundary (see Materials and Methods) were immunostained for BLM and hp150. DAPI staining was for nuclear DNA. Bar, ∼10 μm. (C) Colocalization of BLM and hp150 in S-phase HeLa cells (see Materials and Methods and the Results section for more information). Bar, ∼10 μm.