Table 1.
The 30 most abundant proteins according to emPAI-based molar % identified in corneal amyloid deposits and healthy corneal tissue
| # | A546D/P551Q (Sibling 1) | A546D/P551Q (Sibling 2) | Healthy corneal stroma (3 individuals) | |||
|---|---|---|---|---|---|---|
| Name | Molar % | Name | Molar % | Name | Molar % | |
| 1 | TGFBIp | 69.5 ± 11.3 | TGFBIp | 47.0 ± 7.9 | Collagen alpha-1(I) chain | 36.5 ± 5.8 |
| 2 | Serum amyloid P-component | 3.0 ± 1.2 | Collagen alpha-1(I) chain | 4.1 ± 0.5 | Collagen alpha-2(I) chain | 17.3 ± 1.5 |
| 3 | Apolipoprotein A-I | 2.5 ± 0.6 | Serum amyloid P-component | 3.8 ± 1.0 | TGFBIp | 7.8 ± 2.0 |
| 4 | Collagen alpha-1(I) chain | 2.2 ± 0.4 | Lysozyme C | 3.4 ± 1.5 | Ig kappa chain C region | 5.0 ± 3.0 |
| 5 | Clusterin | 2.2 ± 0.7 | Dermcidin | 2.9 ± 1.9 | Serum albumin | 3.4 ± 0.3 |
| 6 | Collagen alpha-2(I) chain | 2.0 ± 1.0 | Collagen alpha-2(I) chain | 2.6 ± 0.3 | Keratocan | 1.8 ± 0.2 |
| 7 | Serine protease HTRA1 | 1.4 ± 0.8 | Clusterin | 2.5 ± 0.7 | Decorin | 1.6 ± 0.5 |
| 8 | Apolipoprotein A-IV | 1.3 ± 0.5 | Lactotransferrin | 2.0 ± 0.6 | Lumican | 1.4 ± 0.3 |
| 9 | Dermcidin | 1.1 ± 0.6 | Apolipoprotein A-IV | 1.7 ± 0.5 | Ig kappa chain V-III region SIE | 1.3 ± 0.4 |
| 10 | Apolipoprotein D | 0.8 ± 0.4 | Serine protease HTRA1 | 1.5 ± 0.6 | Collagen alpha-3(VI) chain | 1.2 ± 0.2 |
| 11 | Apolipoprotein E | 0.8 ± 0.1 | Thioredoxin | 1.1 ± 0.2 | Ig gamma-1 chain C region | 1.1 ± 0.2 |
| 12 | Protein S100-A8 | 0.7 ± 0.5 | Apolipoprotein E | 1.0 ± 0.3 | Ig gamma-3 chain C region | 1.1 ± 0.3 |
| 13 | Serum albumin | 0.6 ± 0.2 | Complement component C9 | 1.0 ± 0.2 | Collagen alpha-1(VI) chain | 0.9 ± 0.1 |
| 14 | Lysozyme C | 0.6 ± 0.4 | Apolipoprotein A-I | 1.0 ± 0.3 | Ig lambda-2 chain C regions | 0.8 ± 0.4 |
| 15 | Prostaglandin-H2 D-isomerase | 0.5 ± 0.3 | Apolipoprotein D | 0.9 ± 0.2 | Ig kappa chain V-III region NG9 | 0.8 ± 0.4 |
| 16 | Lumican | 0.5 ± 0.2 | Fatty acid-binding protein, epidermal | 0.8 ± 0.2 | Aldehyde dehydrogenase, dimeric NADP-preferring | 0.7 ± 0.1 |
| 17 | Protein S100-A9 | 0.4 ± 0.2 | Actin, cytoplasmic 1 | 0.8 ± 0.2 | Collagen alpha-2(VI) chain | 0.7 ± 0.2 |
| 18 | Ig gamma-1 chain C region | 0.4 ± 0.1 | Vimentin | 0.7 ± 0.2 | Vimentin | 0.7 ± 0.1 |
| 19 | Histone H4 | 0.4 ± 0.1 | Cystatin-A | 0.7 ± 0.4 | Collagen alpha-1(XII) chain | 0.6 ± 0.1 |
| 20 | Ubiquitin-40S ribosomal protein S27a | 0.4 ± 0.3 | Ig gamma-1 chain C region | 0.6 ± 0.2 | Collagen alpha-2(V) chain | 0.6 ± 0.2 |
| 21 | Complement component C9 | 0.4 ± 0.1 | Olfactomedin-like protein 3 | 0.6 ± 0.2 | Ig gamma-4 chain C region | 0.5 ± 0.1 |
| 22 | Olfactomedin-like protein 3 | 0.3 ± 0.1 | Protein S100-A6 | 0.6 ± 0.1 | Prostaglandin-H2 D-isomerase | 0.5 ± 0.2 |
| 23 | Angiopoietin-related protein 7 | 0.3 ± 0.2 | Cytokine-like protein 1 | 0.6 ± 0.2 | MAM domain-containing protein 2 | 0.4 ± 0.1 |
| 24 | Keratocan | 0.3 ± 0.1 | Ubiquitin-40S ribosomal protein S27a | 0.6 ± 0.3 | Histone H4 | 0.4 ± 0.1 |
| 25 | Decorin | 0.3 ± 0.2 | Histone H4 | 0.5 ± 0.1 | Dermcidin | 0.4 ± 0.1 |
| 26 | Ig alpha-1 chain C region | 0.3 ± 0.1 | Protein S100-A8 | 0.5 ± 0.1 | Collagen alpha-1(V) chain | 0.4 ± 0.1 |
| 27 | Phospholipase A2, membrane associated | 0.2 ± 0.1 | Ig kappa chain V-III region VG | 0.5 ± 0.1 | Protein S100-A6 | 0.4 ± 0.2 |
| 28 | Cytokine-like protein 1 | 0.2 ± 0.1 | Glyceraldehyde-3-phosphate dehydrogenase | 0.5 ± 0.3 | Ig alpha-1 chain C region | 0.4 ± 0.2 |
| 29 | Fatty acid-binding protein, epidermal | 0.2 ± 0.1 | Prostaglandin-H2 D-isomerase | 0.5 ± 0.2 | Alpha-enolase | 0.3 ± 0.1 |
| 30 | Actin, cytoplasmic 1 | 0.2 ± 0.1 | Metalloproteinase inhibitor 3 | 0.5 ± 0.2 | Collagen alpha-1(III) chain | 0.3 ± 0.1 |