FIG. 6.
PI3K-stimulated overgrowth is unaffected by mutants of the N-terminal inhibitory site of Sgg. Overgrown eye phenotypes induced by GMR-Gal4-driven expression of activated PI3K-CAAX constructs in sgg mutant backgrounds are shown. The upper row shows eyes from wild-type (WT), sgg+, S9A−, and S9E-sgg rescued flies. The lower row shows overgrown eyes resulting from superimposed PI3K-CAAX-driven expression. The eyes of flies rescued by wild-type or mutant minigenes are of normal size, and they all showed similarly enlarged ommatidial units and eyes after driven expression of PI3K-CAAX. Flies in the sggb12 background are shown, but identical results were obtained in the sggD127 background.