FIG. 2.
Rpd3 regulates internal late-firing origins. Wild-type (OAy618) and rpd3Δ (OAy781) cells were synchronized in late G1 at 23°C with α-factor and released into S phase at 18°C. At the indicated intervals, cells were fixed for analysis. (A) 2-D agarose gel electrophoresis analysis of ARS305, ARS1, ARS603, ARS1413, and ARS319. An open arrow indicates a bubble arc, and a Y arc is marked with a filled arrow. (B) DNA content was analyzed by FACScan. (C) The percentage of budded cells was determined by microscopic analysis of at least 100 cells at each time point.