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. 2014 Sep 15;8:313. doi: 10.3389/fnbeh.2014.00313

Figure 5.

Figure 5

Injection of the translation blocker emetine before conditioning had no effect on 24 h memory retention. Bees received a saline or emetine injection 30 min before single- or three-trial conditioning. Memory retention was tested 24 h after conditioning. ***P < 0.001. (A,B) Averaged performance of all bees; (C,D) averaged performance of bees which showed a conditioned response (CR) during training. (A) The percentage of bees which showed a CR in the 24 h memory retention test differed between single-trial and three-trial conditioning [F(1, 289) = 45, p < 0.001; Two-Way ANOVA] but not between emetine- and saline-injected bees [F(1, 289) = 0.9, p = 0.34]. (B) The discrimination index (DI) of the 24 h memory retention test neither differed between single- and three-trial conditioning [F(1, 289) = 3.3, p = 0.07] nor between emetine- and saline-injected bees [F(1, 289) = 0.2, p = 0.66]. (C) The percentage of bees which showed a CR in the 24 h memory retention test differed between single- and three-trial conditioning [F(1, 191) = 21.2, p < 0.001] but not between emetine- and saline-injected bees [F(1, 191) = 0.1, p = 0.8]. (D) The DI of the 24 h memory retention test neither differed between single- and three-trial conditioning [F(1, 191) = 0.2, p = 0.7] nor between emetine- and saline-injected bees [F(1, 191) = 0.2, p = 0.6].