Table 1.
Purification of recombinant E. coli L-asparaginase II from IBs (culture volume 1 liter).
| Fractions | Total protein (mg) | Specific activity (IU/mg)a | Fold purificationb | % Yield |
|---|---|---|---|---|
| Total cell protein | 580 | – | – | – |
| IBs | 118 | – | – | 100 |
| Solubilized protein | 110 | – | – | 93.2 |
| Refolded protein | 97 | 125 | 1 | 82.2 |
| DEAE ion-exchange chromatography | 78 | 160 | 1.3 | 66.1 |
| Gel filtration chromatography | 60 | 190 | 1.5 | 50.8 |
a
One international unit is defined as amount of enzyme required to produce one micromole of ammonia per min under standard condition of reaction.
b
Fold purification was calculated after refolding as the ratio of specific activity at given step of purification to specific activity of refolded sample.