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Cdx1 interacts with LEF1 in vivo. Whole-cell lysates from Cos7 cells transfected with the Cdx1-Flag and/or Lef1-HA expression vectors were immunoprecipitated with anti-HA antibody and analyzed by Western blotting with an anti-Flag antibody (top panel). Expression of LEF1 was assessed by reprobing the blot with an anti-HA antibody (bottom panel). Inputs represent 5% of the total lysate used for immunoprecipitation.
