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Stage-specific attenuation of Cdx1 expression in Cdx1+/− Wnt3avt/vt compound mutants. Cdx1 expression was assessed by whole-mount in situ hybridization in E8.5 (A to D) or E7.5 (E and F) embryos. (A and E) Wild-type control. (B) Cdx1+/−. (C) Wnt3avt/vt. (D and F) Cdx1+/− Wnt3avt/vt. Note the nearly complete loss of Cdx1 expression at E8.5 in Cdx1+/− Wnt3avt/vt embryos relative to that in the cognate mutant backgrounds, while expression is unaffected at E7.5.
