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. 2014 Sep 15;5:450. doi: 10.3389/fpls.2014.00450

Figure 4.

Figure 4

Conservation protein profile of the M. larici-populina CPG5464 family and AvrP4 homologs in Melampsoraceae. The profile was designed using WebLogo with 40 sequences corresponding to the 12 members in the CPG5464 family (Hacquard et al., 2012), six variants deduced from the 15 genomes sequenced in this study, 22 AvrP4 homologs sequenced from 9 Melampsora spp. (Van der Merwe et al., 2009) and 16 Melampsora lini AvrP4 variants. The predicted signal peptide and K/R and D/E rich regions previously shown in Hacquard et al. (2012) are depicted on the profile. Green arrows point to sites under selection in Barrett et al. (2009). Red arrows point to sites of substitution observed in M. larici-populina variants. Asterisks in the red box indicate amino acids under positive selection in Van der Merwe et al. (2009) and asterisks in the blue box indicate amino acids under positive selection in Hacquard et al. (2012).