FIG. 4.
Theoretical migration of the peptides containing tyrosines 221 and 570 on 2-D peptide maps. The theoretical migrations of peptides containing tyrosines 221 (IQDpYHILTR and IQDpYHILpTR) and 570 (EVGDpYGQLHK and REVGDpYGQLHK) in 2-D peptide maps were calculated based on the parameters of Boyle et al. (8) for TLE at pH 1.9 and thin layer chromatography in phospho-chromatography buffer.