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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Reprod Toxicol. 2012 Aug 31;34(4):512–521. doi: 10.1016/j.reprotox.2012.08.005

Figure 1. Diagram of the ERΔ3 construct used to generate the transgenic mice.

Figure 1

The exons encoding the mERα are numbered according to the coding exons only. The mouse ERα (mERα) cDNA sequences are designated with white bars and noting the loss of exon 3 (Δ3) sequences which correspond to exon 3 of human ERα. The sequences from the pKCR2 vector [38] include the two rabbit β-globin exons (black bars) and one intron (first dark gray bar between exons) as well as 3′ processing signals (second dark gray bar after mERα sequences). The mERα cDNA sequences were inserted into the second β-globin exon. The rat osteocalcin promoter sequences (Osc) are designated as a light gray bar. The enhancer sequences (en) from the U3 LTR of the Harvey murine sarcoma virus (HaMuSV) are indicated by the medium gray bar. The direction of transcription is noted from within the osteocalcin promoter by the arrow. The SV40 polyadenylation signals are located within the pKCR2 sequences. The region of the construct used for expression analysis by RPA is also indicated. The forward (F) and reverse (R) primers used for genotyping are noted. The thin bars at each end of the construct represent the plasmid sequences which are removed prior to microinjection into the FVB/N embryos.