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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Reprod Toxicol. 2012 Aug 31;34(4):512–521. doi: 10.1016/j.reprotox.2012.08.005

Figure 2. Comparison of transgene expression in multiple tissues of adult ERΔ3 male and female mice and detection of ERα protein in WT and ERΔ3 female mice.

Figure 2

Total RNA was analyzed for ERΔ3 transgene transcripts by real-time RT-PCR from tissues dissected from a line D and line F female and line F male mouse. ΔCT value is shown for each tissue based on normalization to the cyclophilin A (Ppia) gene. Lower ΔCT values indicate higher expression levels. Expression relative to ERα and ERβ for these tissues is shown in Table 2. A. Comparisons between female mice for lines D and F are shown for ERΔ3 expression levels (n=1). B. Relative expression between male and female line F mice is indicated for the ERΔ3 transgene (n=1). Gonads represent the ovaries for the female and testes for the male mice. C. A protein in the appropriate size range for the ERΔ3 variant was detected by western blot analysis in the uteri of line D and F ERΔ3 mice in estrus using an antibody directed against the carboxy-terminus of ERα (MC-20). The images for the two uteri each from 3-month-old WT (FVB/N), line F, and line D mice were obtained from the same 10% gel and blot. The band for WT ERα is noted by the upper arrow at 66 kDa and the lower arrow corresponds to the smaller size expected for the ERΔ3 variant (approximately 61–62 kDa). The 70 kDa marker is indicated at the line marked with 70.