Table 2.
Relative Transcript Levels of ERΔ3 to ERα and ERβ in Tissues from Lines D and F Adult Transgenic Mice
| Line/Sex | Tissue | RNA Detection Method | Relative to ERα | Relative to ERβ |
|---|---|---|---|---|
| Line D ♀ | Adrenals | RT-PCR | ↑ (3:1) | ↑ (1400:1) |
| Bone (skull) | RT-PCR | ↑ (10:1) | ↑ (5200:1) | |
| Kidney | RPA | ↑ (9:1) | nd | |
| Liver | RT-PCR & RPA | ↑ (4:1 & 5:1) | ↑a | |
| Lung | RPA | ↑ (18:1) | nd | |
| Mammary gland | RT-PCR & RPA | ↑ (14:1 & 7:1) | ↑ (15800:1) | |
| Ovary | RT-PCR | = (0.7:1) | = (0.7:1) | |
| Uterus | RT-PCR & RPA | ↓ (0.1:1 & 0.4:1) | ↑ (100:1) | |
| Line F ♀ | Adipose | RT-PCR | ↓ (0.5:1) | ↑ (1500:1) |
| Adrenals | RT-PCR | ↑ (2:1) | ↑ (2400:1) | |
| Aorta | RT-PCR | = (1:1) | ↑a | |
| Bone (skull) | RT-PCR | ↑ (3:1) | ↑a | |
| Brain | RT-PCR | ↑ (23:1) | ↑ (110:1) | |
| Kidney | RT-PCR & RPA | ↑ (33:1 & 5:1) | ↑ (8500:1) | |
| Liver | RT-PCR & RPA | ↓ (0.2:1 & 0.2:1) | ↑a | |
| Lung | RPA | ↑ (6:1) | nd | |
| Mammary gland | RT-PCR & RPA | ↓ (0.6:1 & 0.6:1) | ↑ (4100:1) | |
| Ovary | RT-PCR | = (0.8:1) | = (1:1) | |
| Pituitary | RT-PCR | ↓ (0.1:1)b | nd | |
| Uterus | RT-PCR & RPA | ↓ (0.1:1c & 0.7:1) | ↑ (190:1) | |
| Line F ♂ | Bone (skull) | RT-PCR | ↑ (3:1) | ↑ (1500:1) |
| Brain | RT-PCR | ↑ (39:1) | ↑ (280:1) | |
| Kidney | RT-PCR | ↑ (23:1) | ↑ (30100:1) | |
| Liver | RT-PCR | = (0.9:1) | ↑ (2100:1) | |
| Testes | RT-PCR | ↑ (3:1) | ↑ (2300:1) |
a
ERβ not detected by real-time RT-PCR
b
n=3 versus n=1 for other tissues
c
n=6
RT-PCR: real-time RT-PCR (reverse transcriptase-polymerase chain reaction); for ERΔ3, ERα, and ERβ RPA: ribonuclease protection assay; for ERΔ3 and ERα on a different female mouse than for RT-PCR nd: not determined (tissue not examined for ERβ expression)
↑, ERΔ3 levels higher than ERα/ERβ; =, ERΔ3 levels are similar to ERα/ERβ; ↓, ERΔ3 levels lower than ERα