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. 2004 Jun;24(11):5050–5059. doi: 10.1128/MCB.24.11.5050-5059.2004

FIG. 2.

FIG. 2.

Introduction of a human Ku86 cDNA into Ku86-heterozygous cells results in partial restoration of telomere length. (A) Whole-cell extracts were prepared from the indicated cell lines and subjected to immunoblot analysis with commercial monoclonal antibodies directed against either Ku86 or β-actin. The signals on the autoradiogram were quantitated by densitometry, and the ratio of the level of Ku86 to β-actin (K/β) expression is shown below each lane. (B) Genomic DNA was purified from the indicated cell lines and subjected to TRF analysis by denaturing gel electrophoresis. Lanes: 1, HCT116 parental cell line; 2 and 4, clone A6 cells harvested after 30 or 60 population doublings, respectively; 3, HCT116 Ku86-heterozygous cell line #70; 5 and 6, clone A10 cells harvested after 30 or 60 population doublings, respectively; 7 and 8, clone C7 and C5 cell lines (HCT116 Ku86+/− cell lines complemented with an empty vector), respectively. Approximate molecular size markers are shown on the far right.