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PPARγ2, C/EBPα, and C/EBPβ induce adipogenesis in Tet-VP16 control fibroblasts. Tet-VP16 cells were infected and manipulated as described for Fig. 2. (A) Northern blot showing levels of aP2 and adipsin mRNA. Ethidium bromide staining of rRNA is shown as a control. (B) RT-PCR showing levels of PPARγ2 and C/EBPα mRNA. HPRT mRNA is shown as a control. (C) Western blot showing levels of FLAG-tagged dominant-negative BRG1, C/EBPβ, and PI3-kinase.
