FIG. 5.

ChIP analysis of interactions on the PPARγ2 promoter as a function of time of differentiation. B22 cells were infected with a retroviral vector encoding C/EBPα, grown in the presence or absence of tetracycline, and differentiated. One percent of input is shown for each experiment. Portions of the PPARγ2 promoter or the β-actin 5′ untranslated region and coding sequence were amplified from each sample. (A) Levels of interactions with C/EBPα, -β, and -δ. (B) Levels of interactions with tetra-acetylated H4 and K9-, K14-diacetylated H3. (C) Levels of interactions with total Brg1, dominant-negative, FLAG-tagged BRG1, and Ini1. (D) Levels of interactions with TBP, TFIIB, TFIIH p89, and Pol II phosphorylated on Ser-5 of the CTD. Inset: linearity controls for PCR amplifications. Each ChIP was repeated in two to five independent experiments. The data shown in parts A, B, and C derive from a single differentiation experiment. ChIPs in part D derive from a different differentiation experiment.