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The rate of PPARγ transcription decreases after day 4 of 3T3-L1 differentiation. Nuclear run-on assays were performed on nuclei isolated from differentiating 3T3-L1 cells on the days indicated. Run-on transcripts were hybridized to 3T3-L1 genomic DNA, linearized pBABE vector, linearized pBABE containing the PPARγ2 cDNA, or PCR products corresponding to Id1 or Hprt cDNAs.
