FIG. 5.

Representative examples of chromosomal instability on metaphases from an unstable clone, C-32, surviving 10 Gy of X-radiation. Metaphases were hybridized with labeled whole-chromosome probes specific for chromosome 4 (biotinylated and digoxigenin labeled, yellow), chromosome 13 (digoxigenin labeled, red), or chromosome 16 (biotinylated labeled, green). (a) Subpopulation with normal karyotype, containing two copies of chromosome 4, 13, and 16. (b to f) Different subpopulations of metaphase chromosomes showing different kinds of rearrangements (arrowhead).